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ns8593  (Alomone Labs)


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    Structured Review

    Alomone Labs ns8593
    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
    Ns8593, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 11 article reviews
    ns8593 - by Bioz Stars, 2026-02
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    Images

    1) Product Images from "STIM-IP3R crosstalk regulates migration of breast cancer cells"

    Article Title: STIM-IP3R crosstalk regulates migration of breast cancer cells

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.202411203

    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), NS8593 at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
    Figure Legend Snippet: Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), NS8593 at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .

    Techniques Used: Migration, Inhibition, Control, Concentration Assay, Fluorescence, Two Tailed Test



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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), <t>NS8593</t> at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .
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    Image Search Results


    Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), NS8593 at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .

    Journal: The Journal of Cell Biology

    Article Title: STIM-IP3R crosstalk regulates migration of breast cancer cells

    doi: 10.1083/jcb.202411203

    Figure Lengend Snippet: Deletion of STIM proteins sensitizes cell migration to IP3R inhibition. (A) Indicated compounds were added to either WT or dKO cells, and the number of migrating cells from each group was normalized to that of control (DMSO-treated cells). The following inhibitor concentrations were used: 2-APB at 50 μM (WT, n = 5; dKO, n = 11), nifedipine at 50 μM (WT, n = 5; dKO, n = 10), SKF96365 at 10 μM (WT, n = 5; dKO, n = 10), Xes-C at 5 μM (WT, n = 10; dKO, n = 5), NS8593 at 5 μM (WT, n = 5; dKO, n = 5), and ML-9 at 10 μM (WT, n = 5; dKO, n = 5). Bars show the mean ± SEM. (B) Migration was analyzed as in A using the indicated concentration of Xes-C (WT, n = 11–16; dKO, n = 5–11). (C) (C-left) Representative images (left) of anti-paxillin (red) and phalloidin (F-actin, green) fluorescence in dKO cells treated with Xes-C or DMSO (control). (C-right) Mean ± SEM of FAs per cell in WT (control, n = 9; Xes-C, n = 15) or dKO (control, n = 8; Xes-C, n = 13) cells. Scale bar = 10 μm. (D) Migration analysis (bars show the mean ± SEM) of WT ( n = 14), S1KO ( n = 11), S2KO ( n = 10), or dKO cells ( n = 15) treated with the indicated ITPR siRNAs. (E) WB analysis using antibodies for IP3R1, IP3R2, IP3R3, or tubulin of lysates prepared from WT cells treated with siRNAs against IP3R1, IP3R2, or IP3R3, as indicated. Statistics: two-tailed t test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: .

    Article Snippet: In experiments shown in , the following compounds were added to cells: 2-APB (Tocris), nifedipine (Alomone), SKF96365 (Alomone), Xes-C (Tocris), NS8593 (Alomone), and ML-9 (Tocris).

    Techniques: Migration, Inhibition, Control, Concentration Assay, Fluorescence, Two Tailed Test